Influence of Cholesterol Concentration on Bacteriorhodopsin Photocycle
DOI:
https://doi.org/10.15407/ujpe65.9.778Keywords:
laser photolysis, lipid membranes, photocycle, bacteriorhodopsin, cholesterolAbstract
The photocycle of the membrane protein bacteriorhodopsin in Dipalmitoylphosphatidylcholine(DPPC)/Cholesterol membranes with various cholesterol concentrations has been studied using the time-resolved spectroscopy method. The temperature dependences of the rate constants of bacteriorhodopsin transitions between transient states are shown to satisfy the Eyring equation. It is proved that the growth of the cholesterol concentration in the DPPC membrane accelerates the bacteriorhodopsin photocycle.
References
L.A. Bulavin, D.V. Soloviov, Yu.E. Gorshkova et al. Structural transition in a lipid-water liquid system. Ukr. J. Phys. 57, No. 6, 623 (2012).
J. Eisenbl¨atter, R.Winter. Pressure effects on the structure and phase behavior of dmpc-gramicidin lipid bilayers: A synchrotron SAXS and 2H-NMR spectroscopy study. Biophys. J. 90, 956 (2006). https://doi.org/10.1529/biophysj.105.069799
C. Bernsdorff, A. Wolf, R. Winter et al. Effect of hydrostatic pressure on water penetration and rotational dynamics in phospholipid-cholesterol bilayers. Biophys. J. 72, 1265 (1997). https://doi.org/10.1016/S0006-3495(97)78773-3
D.V. Soloviov, L.A. Bulavin, V.I. Gordeliy et al. Neutron scattering investigations of the lipid bilayer structure pressure dependence. Nucl. Phys. At. Energ. 13, No. 1, 83 (2012).
D.V. Soloviov, Y.E. Gorshkova, O.I. Ivankov et al. Ripple phase behavior in mixtures of DPPC/POPC lipids: SAXS and SANS studies. J. Phys. Conf. Ser. 351, 012010 (2012). https://doi.org/10.1088/1742-6596/351/1/012010
D. Soloviov, Y. Zabashta, L. Bulavin et al. Changes in the area per lipid molecule by P-V-T and SANS investigations. Macromol. Symp. 335, 58 (2014). https://doi.org/10.1002/masy.201200122
Y.E. Gorshkova, A.I. Kuklin,V.I. Gordeliy. Structure and phase transitions of DMPC multilamellar vesicles in the presence of Ca2+ ions. J. Surf. Investig. X-ray Synchrot. Neutr. Techn. 11, 27 (2017). https://doi.org/10.1134/S1027451016050499
L.A. Bulavin, D.V. Soloviov, A.I. Kuklin et al. Small-angle X-ray scattering and differential scanning calorimetry studies of DPPC multilamellar structures containing membranotropic agents of different chemical nature. Ukr. J. Phys. 60, 905 (2015).
L.A. Bulavin, D.V. Soloviov, V.I. Gordeliy et al. Lyotropic model membrane structures of hydrated (DPPC: DSC and small-angle X-ray scattering studies of phase transitions in the presence of membranotropic agents. Phase Trans. 88, 582 (2015). https://doi.org/10.1080/01411594.2014.1002784
A.O. Krasnikova, N.A. Kasian, O.V. Vashchenko et al. Effect of oxyethilated glycerol cryoprotectants on DPPC model lipid membranes structure and phase. Probl. Cryobiol. Cryomed. 25, No. 2, 186 (2015). https://doi.org/10.15407/cryo25.02.186
O.V. Vashchenko, S.V. Shishkina, N.A. Kasian et al. Formation of antibiotic cycloserine complexes with stearic acid and its calcium and magnesium salts: From quantum mechanical modeling to studies of membranotropic action. Funct. Mater. 26, 673 (2019). https://doi.org/10.15407/fm26.04.673
M. Zhernenkov, D. Bolmatov, D. Soloviov et al. Revealing the mechanism of passive transport in lipid bilayers via phonon-mediated nanometre-scale density fluctuations. Nature Commun. 7, 11575 (2016). https://doi.org/10.1038/ncomms11575
D. Soloviov, Y.Q. Cai, D. Bolmatov et al. Functional lipid pairs as building blocks of phase-separated membranes. Proc. Natl. Acad. Sci. U.S.A. 117, 4749 (2020). https://doi.org/10.1073/pnas.1919264117
M. Bogdanov, E. Mileykovskaya, W. Dowhan et al. Lipids in the assembly of membrane proteins and organization of protein supercomplexes: Implications for lipid-linked disorders. In: Lipids in Health and Disease. Edited by P.J. Quinn, Xiaoyuan Wang (Springer, 2008), SCBI, 49 pp. 197-239. https://doi.org/10.1007/978-1-4020-8831-5_8
O.I. Ivankov, E.V. Ermakova, T.N. Murugova et al. Interactions in the Model Membranes Mimicking Preclinical Conformational Diseases (Elsevier, 2020) [ISBN: 2451-9634]. https://doi.org/10.1016/bs.abl.2020.02.002
N. Ntarakas, I. Ermilova, A.P. Lyubartsev et al. Effect of lipid saturation on amyloid-beta peptide partitioning and aggregation in neuronal membranes: Molecular dynamics simulations. Eur. Biophys. J. 48, 813 (2019). https://doi.org/10.1007/s00249-019-01407-x
M. Javanainen, H. Martinez-Seara, I. Vattulainen. Nanoscale membrane domain formation driven by cholesterol. Sci. Rep. 7, 1143 (2017). https://doi.org/10.1038/s41598-017-01247-9
B.L. Stottrup, S.L. Keller. Phase behavior of lipid monolayers containing DPPC and cholesterol analogs. Biophys. J. 90, 3176 (2006). https://doi.org/10.1529/biophysj.105.072959
J.H. Ipsen, G. Karlstr¨om, O. Mourtisen et al. Phase equilibria in the phosphatidylcholine-cholesterol system. Biochim. Biophys. Acta 905, 162 (1987). https://doi.org/10.1016/0005-2736(87)90020-4
O. Edholm, J.F. Nagle. Areas of molecules in membranes consisting of mixtures. Biophys. J. 89, 1827 (2005). https://doi.org/10.1529/biophysj.105.064329
I. Chizhov, D.S. Chernavskii, M. Engelhard et al. Spectrally silent transitions in the bacteriorhodopsin photocycle. Biophys. J. 71, 2329 (1996). https://doi.org/10.1016/S0006-3495(96)79475-4
D. Bratanov, K. Kovalev, J.-P. Machten et al. Unique structure and function of viral rhodopsins. Nature Commun. 10, 4939 (2019). https://doi.org/10.1038/s41467-019-12718-0
I. Chizhov, G. Schmies, R. Seidel et al. The photophobic receptor from Natronobacterium pharaonis: temperature and pH dependencies of the photocycle of sensory rhodopsin II. Biophys. J. 75, 999 (1998). https://doi.org/10.1016/S0006-3495(98)77588-5
I.V. Chizhov. Flash photolysis. In Encyclopedia of Biophysics (Springer, 2013) [ISBN: 978-3-642-16712-6]. https://doi.org/10.1007/978-3-642-16712-6_63
K.H. M¨uller, T. Plesser. Variance reduction by simultaneous multi-exponential analysis of data sets from different experiments. Eur. Biophys. J. 19, 241 (1991). https://doi.org/10.1007/BF00183531
T. Hianik, V.I. Passechnik. Bilayer Lipid Membranes. Structure and Mechanical Properties (Springer Sci. and Business Media, 1995) [ISBN: 0792335511].
Downloads
Published
How to Cite
Issue
Section
License
Copyright Agreement
License to Publish the Paper
Kyiv, Ukraine
The corresponding author and the co-authors (hereon referred to as the Author(s)) of the paper being submitted to the Ukrainian Journal of Physics (hereon referred to as the Paper) from one side and the Bogolyubov Institute for Theoretical Physics, National Academy of Sciences of Ukraine, represented by its Director (hereon referred to as the Publisher) from the other side have come to the following Agreement:
1. Subject of the Agreement.
The Author(s) grant(s) the Publisher the free non-exclusive right to use the Paper (of scientific, technical, or any other content) according to the terms and conditions defined by this Agreement.
2. The ways of using the Paper.
2.1. The Author(s) grant(s) the Publisher the right to use the Paper as follows.
2.1.1. To publish the Paper in the Ukrainian Journal of Physics (hereon referred to as the Journal) in original language and translated into English (the copy of the Paper approved by the Author(s) and the Publisher and accepted for publication is a constitutive part of this License Agreement).
2.1.2. To edit, adapt, and correct the Paper by approval of the Author(s).
2.1.3. To translate the Paper in the case when the Paper is written in a language different from that adopted in the Journal.
2.2. If the Author(s) has(ve) an intent to use the Paper in any other way, e.g., to publish the translated version of the Paper (except for the case defined by Section 2.1.3 of this Agreement), to post the full Paper or any its part on the web, to publish the Paper in any other editions, to include the Paper or any its part in other collections, anthologies, encyclopaedias, etc., the Author(s) should get a written permission from the Publisher.
3. License territory.
The Author(s) grant(s) the Publisher the right to use the Paper as regulated by sections 2.1.1–2.1.3 of this Agreement on the territory of Ukraine and to distribute the Paper as indispensable part of the Journal on the territory of Ukraine and other countries by means of subscription, sales, and free transfer to a third party.
4. Duration.
4.1. This Agreement is valid starting from the date of signature and acts for the entire period of the existence of the Journal.
5. Loyalty.
5.1. The Author(s) warrant(s) the Publisher that:
– he/she is the true author (co-author) of the Paper;
– copyright on the Paper was not transferred to any other party;
– the Paper has never been published before and will not be published in any other media before it is published by the Publisher (see also section 2.2);
– the Author(s) do(es) not violate any intellectual property right of other parties. If the Paper includes some materials of other parties, except for citations whose length is regulated by the scientific, informational, or critical character of the Paper, the use of such materials is in compliance with the regulations of the international law and the law of Ukraine.
6. Requisites and signatures of the Parties.
Publisher: Bogolyubov Institute for Theoretical Physics, National Academy of Sciences of Ukraine.
Address: Ukraine, Kyiv, Metrolohichna Str. 14-b.
Author: Electronic signature on behalf and with endorsement of all co-authors.
.png){kind=small}
