Phosphorenes as a New Material for Surface-Enhanced Infrared Spectroscopy of Cell Membranes
DOI:
https://doi.org/10.15407/ujpe71.4.349Keywords:
phosphorenes, spectral markers, model membranes, surface-enhanced infrared absorptionAbstract
Phosphorene is a promising two-dimensional (2D) material consisting of a single-layer phosphorus artificially created from layered black phosphorus, which is known to be the most stable allotrope of phosphorus. Phosphorene, similar to graphene-based substrates used in surfaceenhanced infrared spectroscopy (SEIRA), is expected to exhibit similar behavior. The results of a SEIRA experiment with a model biological cell membrane, formed on the basis of dioleoylphosphatidylcholine (DOPC) lipids, and deposited onto the surface of several phosphorene layers are presented. The enhancement of the absorption factor by up to 2–4 times was observed without changing the positions of the absorption frequencies of the bands corresponding to the main spectral markers of liposomes, which is comparable to that observed for metallic silver particles. Density functional theory (DFT) calculations of the DOPC-phosphorene system indicate a high reactivity of multilayer phosphorene and a possibility of the rapid, often reversible oxidation and reduction processes depending on the pH of the medium. Taking into account that the optical signal from the membrane is insignificant, this technique can be recommended for the use of two-dimensional phosphorene as SEIRA and SERS substrates for membrane studies.
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