Monitoring of the Enzymatic Reactions Course by Differential Microwave Dielectrometry Method in Real Time
DOI:
https://doi.org/10.15407/ujpe68.9.608Keywords:
high loss liquids, complex permittivity, enzymatic reactions, electromagnetic wave propagation, differential microwave dielectrometry, enzyme trypsin, human serum albumin, immunoglobulin GAbstract
Enzymatic reactions are the basis of many biotechnological manufacturing and biomedical diagnostic procedures that require effective methods of monitoring over the reaction course. In the current paper, we present the results of the development of a new approach within the differential microwave dielectrometry method for the non-invasive monitoring of the course of enzymatic reactions based on the complex permittivity changes of these reactive mixture solutions in real time at a fixed frequency of 31.82 GHz. The dynamic studies of the dielectric parameters of selected enzymatic systems containing a protein substrate (immunoglobulin G, human serum albumin) and enzyme trypsin. The developed differential microwave dielectrometry setup has been performed to verify the proposed approach effectiveness for the enzymatic reaction monitoring in biomedical practice and the food industry. Our microwave dielectrometry results have been validated by the results of the UV-Vis spectrophotometry method for selected enzymatic systems. We propose a new approach to use the differential microwave dielectrometry method with high sensitivity (in average 0.5% and 3÷5% for the real and imaginary parts of the complex permittivity, respectively) to estimate the course of enzymatic reactions in real time.
References
G.D. Najafpour. Biochemical Engineering and Biotechnology (Elsevier, 2015) [ISBN: 978-0-444-63357-6].
https://doi.org/10.1016/B978-0-444-63357-6.00002-X
K.S. Siddiqui, H. Ertan, A. Poljak, W.J. Bridge. Evaluating enzymatic productivity-the missing link to enzyme utility. Int. J. Mol. Sci. 23 (13), 6908 (2022).
https://doi.org/10.3390/ijms23136908
M. Faure, M. Kechadi, B. Sotta, J. Gamby, B. Tribolleta. Contact free impedance methodology for investigating enzymatic reactions into dielectric polymer microchip. Electroanalysis 25 (5), 1151 (2013).
https://doi.org/10.1002/elan.201200488
Y. Iwasaki, T. Horiuchi, O. Niwa. Detection of electrochemical enzymatic reactions by surface plasmon resonance measurement. Anal. Chem. 73 (7), 1595 (2001).
https://doi.org/10.1021/ac0012851
A. Furukawa, T. Nagata, A. Matsugami, Y. Habu, R. Sugiyama, F. Hayashi, N. Kobayashi, S. Yokoyama, H. Takaku, M. Katahira. Structure, interaction and realtime monitoring of the enzymatic reaction of wild-type APOBEC3G. The EMBO J. 28 (4), 440 (2009).
https://doi.org/10.1038/emboj.2008.290
F. C. Church, H. E. Swaisgood, D.H. Porter, G.L. Cattignani. Spectrophotometric assay using o-phthaldialdehyde for determination of proteolysis in milk and isolated milk proteins. J. Dairy Sci. 66, 1219 (1983).
https://doi.org/10.3168/jds.S0022-0302(83)81926-2
W.J. Ellison, K. Lamkaourchi, M.J. Moreau. Water: A dielectric reference. J. Mol. Liq. 68, 171 (1996).
https://doi.org/10.1016/0167-7322(96)00926-9
A. Godio. Open ended-coaxial cable measurements of saturated sandy soils. American J. Environmental Sci. 3 (3), 175 (2007).
https://doi.org/10.3844/ajessp.2007.175.182
J. M. McKee, B.P. Johnson. Real-time chemical sensing of aqueous ethanol glucose mixtures. IEEE Transactions on Instrumentation and Measurement 49 (1), 114 (2000).
https://doi.org/10.1109/19.836320
I. Dilman, M. N. Akinci, T. Yilmaz, M. ¸Cay¨oren, I. Akduman. A method to measure complex dielectric permittivity with open-ended coaxial probes. IEEE Transactions on Instrumentation and Measurement 71, (2022).
https://doi.org/10.1109/TIM.2022.3147878
Keysight Technologies, Keysight n1501a Dielectric Probe Kit 200 MHz to 50 GHz. 2021. [Online]. Available: http://literature.cdn.keysight.com/litweb/pdf/5989-0222EN.pdf.
F. Artis, T. Chen, T. Chretiennot, J.-J. Fournie, M. Poupot, D. Dubuc, K. Grenier. Microwaving biological cells: intracellular analysis with microwave dielectric spectroscopy. IEEE Microwave Magazine 16 (4), 87 (2015).
https://doi.org/10.1109/MMM.2015.2393997
K. Shibata. Measurement of complex permittivity for liquid materials using the open-ended cut-off waveguide reflection method. IEICE Transactions on Electronics E93-C (11), 1621 (2010).
https://doi.org/10.1587/transele.E93.C.1621
Y. Wang, M.N. Afsar. Measurement of complex permittivity of liquids using waveguide techniques. Progress in Electromagnetics Research 42, 131 (2003).
https://doi.org/10.2528/PIER03010602
J. Barthel, K. Bachhuber, R. Buchner, H. Hetzenauer. Dielectric spectra of some common solvents in the microwave region. Water and lower alcohols. Chem. Phys. Lett. 165 (4), 369 (1990).
https://doi.org/10.1016/0009-2614(90)87204-5
Z.E. Eremenko, K.S. Kuznetsova, N.I. Sklyar, A.V. Martynov. Measuring Complex Permittivity of High-Loss Liquids in Dielectric Materials and Applications. Edited by P.G. Choudhry (Nova Science Publishers, 2019), Chap. 2 [ISBN: 978-1-53615-316-3].
Z.E. Eremenko, V.N. Skresanov, A.I. Shubnyi, N.S. Anikina, V.G. Gerzhikova, T.A. Zhilyakova. Complex Permittivity Measurement of High Loss Liquids and its Application to Wine Analysis in Electromagnetic Waves. Edited by V. Zhurbenko (Janeza Trdine, 2011) [ISBN: 978-953-307-304-0].
Z.E. Eremenko, V.A. Pashynska, K.S. Kuznetsova, O.I. Shubnyi, N.I. Sklyar, A.V. Martynov. Microwave dielectrometer application to antibiotic concentration control in water solution. Low Temperature Physics 26, (3), 30 (2021).
https://doi.org/10.15407/rej2021.03.030
V.N. Skresanov, Z.E. Eremenko, E.S. Kuznetsova, Y. Wu, Y. He. Circular layered waveguide use for wideband complex permittivity measurement of lossy liquids. IEEE Transactions on Instrumentation and Measurement 63 (3), 694 (2014).
https://doi.org/10.1109/TIM.2013.2282003
S. Schmidt, M. Schubler. All Liquid Based Calibration Scheme for Microwave Dielectrometry. In Proceedings IEEE/MTT-S International Microwave Symposium USA, 8058839 (2017).
https://doi.org/10.1109/MWSYM.2017.8058839
A.A. Abduljabar, D.J. Rowe, A. Porch, D.A. Barrow. Novel microwave microfluidic sensor using a microstrip split-ring resonator. IEEE Transactions on Microwave Theory and Techniques 62 (3), 679 (2014).
https://doi.org/10.1109/TMTT.2014.2300066
A.I. Gubin, A.A. Barannik, N.T. Cherpak, I.A. Protsenko, S. Pud, A. Offenh¨ausser, S.A. Vitusevich. Whisperinggallery-mode resonator technique with microfluidic channel for permittivity measurement of liquids. IEEE Transactions on Microwave Theory and Techniques 63 (6), 2003 (2015).
https://doi.org/10.1109/TMTT.2015.2423289
J.-W.C. Alffenaar, E.M. Jongedijk, C.A.J. van Winkel, M. Sariko, S.K. Heysell, S. Mpagama, D.J. Touw. A mobile microvolume UV/visible light spectrophotometer for the measurement of levofloxacin in saliva. J. Antimicrobial Chemotherapy 76 (2), 423 (2021).
https://doi.org/10.1093/jac/dkaa420
Huffman, K. Soni, J. Ferraiolo. UV-Vis based determination of protein concentration: validating and implementing slope measurements using variable pathlength technology. BioProcess International 12 (8), (2014).
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