Small-Angle Neutron Scattering Study of Bicelles and Proteobicelles with Incorporated Mitochondrial Cytochrome c Oxidase


  • K. Siposova Institute of Experimental Physics, Slovak Academy of Sciences
  • V. I. Petrenko BCMaterials, Basque Centre for Materials, Applications and Nanostructures, IKERBASQUE, Basque Foundation for Science
  • O. I. Ivankov Joint Institute for Nuclear Research, Institute for Safety Problems of Nuclear Power Plants, Nat. Acad. of Sci. of Ukraine
  • L. A. Bulavin Taras Shevchenko National University of Kyiv
  • A. Musatov Institute of Experimental Physics, Slovak Academy of Sciences



small-angle neutron scattering, bicelles, cytochrome c oxidase, aggregation state


The structural investigations of a model membrane system, bicelles, and the aggregation state of isolated and purified bovine heart cytochrome c oxidase (CcO) in bicelles have been performed using small-angle neutron scattering (SANS), SANS contrast variation, and complemented by various biophysical and biochemical techniques. The average size of bicelles prepared from long-chain 1,2-dimyristoyl-sn-glycero-3-phosphocholine and short-chain 1,2-dihexanoyl-sn-glycero-3-phosphocholine was found to be about 22 nm with a thickness of about 4 nm. Enzyme in bicelles was remained active and structurally unaltered. The estimated volume of protein in bicelles of 240 nmcorresponded well to the monomeric form of CcO. The ab initio modeling supports the experimental data and suggests that CcO in bicelles is a homogeneous monomeric complex incorporated into bicelles.


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How to Cite

Siposova, K., Petrenko, V. I., Ivankov, O. I., Bulavin, L. A., & Musatov, A. (2020). Small-Angle Neutron Scattering Study of Bicelles and Proteobicelles with Incorporated Mitochondrial Cytochrome c Oxidase. Ukrainian Journal of Physics, 65(8), 662.



Physics of liquids and liquid systems, biophysics and medical physics

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