Experimental Approach to the Creation of Efficient Multicomponent Nanocomposites for Antitumor Therapy
DOI:
https://doi.org/10.15407/ujpe65.8.678Keywords:
polymer nanocarrier, gold nanoparticles, Chlorine e6, Doxorubicin, antitumor therapyAbstract
Water-soluble polymers with special characteristics can be used as carriers in which the active ingredients are entrapped, encapsulated, adsorbed, or chemically attached. The understanding of the processes occurring during the formation of multicomponent nanosystems is the urgent task for the synthesis of antitumor nanocomposites. Gold nanoparticles (AuNPs), photosensitizer Chlorine e6 (Ce6), and Doxorubicin (Dox) are currently used for the photodynamic therapy and chemotherapy. We have been focused on the study of three-component nanosystems Polymer/AuNPs/Ce6, and four-component nanosystems Polymer/AuNPs/Ce6/Dox at physiological temperatures (37 ∘C). The star-like copolymer with Dextran core and grafted Polyacrylamide chains in nonionic and anionic forms are used as a matrix for the synthesis of nanocomposites. The nanosystems are characterized by the dynamic light scattering and transmission electron microscopy. The increasing of the aggregation processes for the four-component nanosystem Polymer/AuNPs/Ce6/Dox in comparison with the three-component one Polymer/AuNPs/Ce6 is registered. These nanosystems are tested in vitro against the subline of breast carcinoma MCF-7/S – sensitive to cytostatics. It is demonstrated that the increase of the aggregation process occurring in four-component systems leads to the loss of the antitumor activity of multicomponent drugs.
References
S. Caban, E. Aytekin, A. Sahin, A.Y. Capan. Nanosystems for drug delivery. OA Drug Design & Delivery 2, 2 (2014).
S.K. Sahoo, V. Labhasetwar. Nanotech approaches to drug delivery and imaging. Drug Discov. Today 8 (24), 1112 (2003). https://doi.org/10.1016/S1359-6446(03)02903-9
N.A. Ochekpe, P.O. Olorunfemi, N.C. Ngwuluka. Nanotechnology and drug delivery part 1: Background and applications. Trop. J. Pharm. Res. 8 (3), 265 (2009). https://doi.org/10.4314/tjpr.v8i3.44546
N.R. Jabir, S. Tabrez, G.M. Ashraf et al. Nanotechnology-based approaches in anticancer research. Int. J. Nanomed. 7, 4391 (2012). https://doi.org/10.2147/IJN.S33838
N. Jawahar, S.N. Meyyanathan. Polymeric nanoparticles for drug delivery and targeting: A comprehensive review. Int. J. Health Allied. Sci. 1, 217 (2012). https://doi.org/10.4103/2278-344X.107832
W. Mei, Q. Wu. Applications of metal nanoparticles in medicine/Metal nanoparticles as anticancer agents. Metal Nanoparticles, ??? 169 (2017). https://doi.org/10.1002/9783527807093.ch7
G. Frens. Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions. Nature Phys. Sci. 241 (105), 20 (1973). https://doi.org/10.1038/physci241020a0
L.A. Dykman, N.G. Khlebtsov. Gold nanoparticles in biology and medicine: recent advances and prospects. Acta Naturae 3 (2), 34 (2011). https://doi.org/10.32607/20758251-2011-3-2-34-55
P.G. Calavia, G. Bruce, L. Perez-Garcia, D.A. Russell. Photosensitiser-gold nanoparticle conjugates for photodynamic therapy of cancer. Photochem. Photobiol. Sci. 17 (11), 1534 (2018). https://doi.org/10.1039/C8PP00271A
N.V. Kutsevol, V.A. Chumachenko, M. Rawiso et al. Star-like dextran-polyacrylamide polymers: Prospects of use in nanotechnologies. J. Struct. Chem. 56 (5), 959 (2015). https://doi.org/10.1134/S0022476615050200
T. Matvienko, V. Sokolova, S. Prylutska et al. In vitro study of the anticancer activity of various Doxorubicin-containing dispersions. Bioimpacts 9 (1), 59 (2019). https://doi.org/10.15171/bi.2019.07
G. Telegeev, N. Kutsevol, V. Chumachenko et al. Dextran-polyacrylamide as matrices for creation of anticancer nanocomposite. Int. J. Pol. Sci.Article ID 4929857, (2017). https://doi.org/10.1155/2017/4929857
N. Kutsevol, A. Naumenko, V. Chumachenko et al. Agregation processes in hybrid nanosystem polymer/nano-silver/cisplatin. Ukr. J. Phys. 63 (6), 513 (2018). https://doi.org/10.15407/ujpe63.6.513
V.A. Chumachenko, I.O. Shton, E.D. Shishko et al. Branched copolymers dextran-graft-polyacrylamide as nanocarriers for delivery of gold nanoparticles and photosensitizers to tumor cells. In: Nanophysics, Nanophotonics, Surface Studies, and Applications (Springer, 2015), pp. 379-390. https://doi.org/10.1007/978-3-319-30737-4_32
M. Bezuglyi, N. Kutsevol, M. Rawiso, T. Bezugla. Water-soluble branched copolymers dextran-polyacrylamide and their anionic derivates as matrices for metal nanoparticles. In-Situ Synthesis, Chemik. 8 (66), 862 (2012).
S. Provencher. CONTIN: A general purpose constrained regularization program for inverting noisy linear algebraic and integral equations. Comput. Phys. Commun. 27, 229 (1992). https://doi.org/10.1016/0010-4655(82)90174-6
A. Scotti, W. Liu, J.S. Hyatt et al. The CONTIN algorithm and its application to determine the size distribution of microgel suspensions. J. Chem. Phys. 142, 234905 (2015). https://doi.org/10.1063/1.4921686
N. Kutsevol, A. Naumenko, V. Chumachenko et al. Agregation processes in hybrid nanosystem polymer/nano-silver/cisplatin. Ukr. J. Phys. 63 (6), 513 (2018). https://doi.org/10.15407/ujpe63.6.513
V. Chumachenko, N. Kutsevol, Yu. Harahuts et al. Star-like Dextran-graft-PNiPAM copolymers. Effect of internal molecular structure on the phase transition. J. Mol. Liquids 235, 77 (2017). https://doi.org/10.1016/j.molliq.2017.02.098
E. Tomaszewska, K. Soliwoda, K. Kadziola, et al. Detection limits of DLS and UV-Vis spectroscopy in characterization of polydisperse nanoparticles colloids. J. Nanomaterials Article ID 313081, 10 pages (2013). https://doi.org/10.1155/2013/313081
Downloads
Published
How to Cite
Issue
Section
License
Copyright Agreement
License to Publish the Paper
Kyiv, Ukraine
The corresponding author and the co-authors (hereon referred to as the Author(s)) of the paper being submitted to the Ukrainian Journal of Physics (hereon referred to as the Paper) from one side and the Bogolyubov Institute for Theoretical Physics, National Academy of Sciences of Ukraine, represented by its Director (hereon referred to as the Publisher) from the other side have come to the following Agreement:
1. Subject of the Agreement.
The Author(s) grant(s) the Publisher the free non-exclusive right to use the Paper (of scientific, technical, or any other content) according to the terms and conditions defined by this Agreement.
2. The ways of using the Paper.
2.1. The Author(s) grant(s) the Publisher the right to use the Paper as follows.
2.1.1. To publish the Paper in the Ukrainian Journal of Physics (hereon referred to as the Journal) in original language and translated into English (the copy of the Paper approved by the Author(s) and the Publisher and accepted for publication is a constitutive part of this License Agreement).
2.1.2. To edit, adapt, and correct the Paper by approval of the Author(s).
2.1.3. To translate the Paper in the case when the Paper is written in a language different from that adopted in the Journal.
2.2. If the Author(s) has(ve) an intent to use the Paper in any other way, e.g., to publish the translated version of the Paper (except for the case defined by Section 2.1.3 of this Agreement), to post the full Paper or any its part on the web, to publish the Paper in any other editions, to include the Paper or any its part in other collections, anthologies, encyclopaedias, etc., the Author(s) should get a written permission from the Publisher.
3. License territory.
The Author(s) grant(s) the Publisher the right to use the Paper as regulated by sections 2.1.1–2.1.3 of this Agreement on the territory of Ukraine and to distribute the Paper as indispensable part of the Journal on the territory of Ukraine and other countries by means of subscription, sales, and free transfer to a third party.
4. Duration.
4.1. This Agreement is valid starting from the date of signature and acts for the entire period of the existence of the Journal.
5. Loyalty.
5.1. The Author(s) warrant(s) the Publisher that:
– he/she is the true author (co-author) of the Paper;
– copyright on the Paper was not transferred to any other party;
– the Paper has never been published before and will not be published in any other media before it is published by the Publisher (see also section 2.2);
– the Author(s) do(es) not violate any intellectual property right of other parties. If the Paper includes some materials of other parties, except for citations whose length is regulated by the scientific, informational, or critical character of the Paper, the use of such materials is in compliance with the regulations of the international law and the law of Ukraine.
6. Requisites and signatures of the Parties.
Publisher: Bogolyubov Institute for Theoretical Physics, National Academy of Sciences of Ukraine.
Address: Ukraine, Kyiv, Metrolohichna Str. 14-b.
Author: Electronic signature on behalf and with endorsement of all co-authors.
.png){kind=small}
