Low-Temperature Luminescent Studies of Emissive Guanine Substitute for the Detection of Biopolymers

  • V. Yu. Kudrya Taras Shevchenko National University of Kyiv
  • V. M. Yashchuk Taras Shevchenko National University of Kyiv
  • A. P. Naumenko Taras Shevchenko National University of Kyiv
  • Y. Mely Laboratoire de Biophotonique et Pharmacologie, Faculte de Pharmacie, UMR 7213 CNRS, Universite de Strasbourg
  • Ya. O. Gumenyuk National University of Life and Environmental Sciences of Ukraine
Keywords: emissive guanine substitute, fluorescence, phosphorescence, energy transfer by excitations

Abstract

The optical absorption at 300 K and the fluorescence and phosphorescence at 78 K of the emissive guanine substitute, deoxythienoguanosine, (dthG) were investigated in aqueous and TRIS-HCl-buffer solutions. Two optical absorption and fluorescence centers at room temperature were attributed to two keto-enol tautomers of dthG, which confirms previously obtained results. In contrast to room temperature, only one emission band was observed at 78 K in fluorescence spectra that was close to the long-wave fluorescence band at room temperature and could be associated with the tautomer with long-wave absorption. This phenomenon can be explained by the energy transfer by excitations in a frozen solution between two types of the optical centers mentioned above. The similar conclusion is drawn for the phosphorescence: only one tautomer phosphorescence band is observed. The spectral positions of this band maximum are essentially different for aqueous and buffer solutions (∼50 nm).

References

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Published
2020-04-17
How to Cite
Kudrya, V., Yashchuk, V., Naumenko, A., Mely, Y., & Gumenyuk, Y. (2020). Low-Temperature Luminescent Studies of Emissive Guanine Substitute for the Detection of Biopolymers. Ukrainian Journal of Physics, 65(4), 317. https://doi.org/10.15407/ujpe65.4.317
Section
Optics, atoms and molecules

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