Investigation of the Kinetics of Photoinduced Electronic Transitions in Nanostructures of Bacterial Reaction Centers
We have studied the slow dynamics of isolated complexes of chlorophyll-containing membrane proteins of photosynthetic reaction centers (RCs) of Rb. sphaeroides R-26 induced by light fluxes. The analysis of a variation of the absorption of a solution of RCs in the frame of the three-level model is carried out. The equation defining the ratio of the populations of the electron levels of the primary and secondary quinones is deduced. The solution of this equation has non-Boltzmann character even in the stationary case and depends on the exciting light intensity. A model of the dynamics of levels of RC, which accounts for the presence of polarization processes in a vicinity of the secondary quinone acceptor (QB) is proposed. It is assumed that all RCs have the same structure, but can be in different states, whose characteristics depend on both the time interval having passed after the absorption of a light quantum and the local viscosity and elasticity of the environment of QB. The presence of deformational properties of the environment of QB yields the possibility for a configuration of RC to be changed, which agrees with the experimental data.
J.M. Olson and J.P. Trornber, Membrane Proteins in Energy Transduction (Marcel Dekker, New York, 1999).
A.B. Rubin, Biophysics (Moscow State Univ., Moscow, 2000), Vol. 2 (in Russian).
M. Amin, L. Vogt, S. Vassiliev, I. Rivalta, M.M. Sultan, D. Bruce, G.W. Brudvig, V.S. Batista, and M.R. Gunner, Electrostatic effects on proton coupled electron transfer in oxomanganese complexes inspired by the oxygen-evolving complex of photosystem II, J. Phys. Chem. 117, 6217 (2013) [DOI: 10.1021/jp403321b].
V.N. Kharkyanen, Yu.M. Barabash, N.M. Berezetskaya, E.P. Lukashev, P.P. Knox, and L.N. Christophorov, Peculiarities of light-induced slow protein dynamics in the photosynthetic reaction center, Chem. Phys. Lett. 512, Nos. 1–3, 113 (2011) [DOI: 10.1016/j.cplett.2011.07.006].
Yu.M. Barabash, N.M. Berezetskaya, L.N. Christophorov, A.O. Goushcha, and V.N. Kharkyanen, Effects of structural memory in protein reactions, J. Chem. Phys. 116, No. 10, 4339 (2002) [DOI: 10.1063/1.1447906].
A.K. Kukushkin and A.N. Tikhonov, Lectures on Biophysics of Photosynthesis (Moscow State Univ., Moscow, 1988) (in Russian).
Yu.M. Barabash, M.A. Zabolotny, N.I. Sokolov, and V.N. Kharkyanen, Slow photoinduced changes in reaction centers of Rhodobacter sphaeroides R-26 by holographic interferometry, Biofiz. 47, No. 6, 970 (2002).
A.O. Goushcha, M.T. Kapoustina, V.N. Kharkyanen, and A.R. Holzwarth, Nonlinear dynamic processes in an ensemble of photosynthetic reaction centers. Theory and experiment, J. Phys. Chem. B 101, No. 38, 7612 (1997) [DOI: 10.1021/jp970868k].
V.I. Gol’dansky, L.I. Trakhtenberg, and V.N. Flerov, Tunneling Phenomena in Chemical Physics, (Nauka, Moscow, 1986) (in Russian).